Hi,
1. Have You asked abt fuel oil or diesel oil ?
a) if fuel oil (IFO)- then separating temperature should be at 90-95 degC.
b)in case of MGO / MDO separation temp. is appx 40 degC appx.
For some reasons - this fact is forgotten 9 of 10 times...
2. Don't forget: Separator can work in two modes: clarifier and purifier.
one eliminates only impurities, another - impurities and water. This can be done by replacing of gravity disc and top disc...
Please also note - separator capacity (throughput) has to be as low as possible (just enough + a bit more to pace up with engine), then it is most effective.
*Regarding Alfa Laval: They make an ALCAP system, or SU- types, both are very advanced.
3. The best solution - clarifier + purifier, what are switched in "series".
4. I agree with K1W1 - if You got small, irritating animals in fuel

USE BIOCIDE !!!

try out Drew, Unitor , Vecom. They all have a solution. My best experience - Drew Ameroid.
5. "Bio" problems are becoming worse, if fuel tank is stand-still (not shaken) and when fuel is "getting older".
6. Do not forget to drain settlings daily !!!
rgds,
Andrei